RNA

Part:BBa_K4811022:Design

Designed by: Kasper Krunderup Jakobsen   Group: iGEM23_DTU-Denmark   (2023-10-03)


PT7-TMS(Theo1)-TT7


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 89
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

As of 2023, the TMS is a novel BioBrick, and not much is known about the specifics of TMS to RBS binding, as well as how an aptamer insertion into the D-loop will alter this binding, both in presence and absence of the ligand.


Source

The part was ordered as a gBlock from IDT.

The TMS was developed by Avishek Paul during their PhD thesis, see the publication "Modular and Versatile Trans-Encoded Genetic Switches", A. Paul, E. M. Warszawik, M. Loznik, A. J. Boersma, A. Herrmann, Angew. Chem. Int. Ed. 2020, 59, 20328.

The Theophylline sensitive aptamer was adapted from Beatrix Suess, Barbara Fink, Christian Berens, Régis Stentz, Wolfgang Hillen, A theophylline responsive riboswitch based on helix slipping controls gene expression in vivo, Nucleic Acids Research, Volume 32, Issue 4, 15 February 2004, Pages 1610–1614, https://doi.org/10.1093/nar/gkh321


References